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Index > Protein center > NOS2(Gene name) > Chicken
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  • NOS2 (Gene name),
  • Nitric oxide synthase, inducible (Protein name ),  NOS2_CHICK from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    NOS2;
    Protein name:
    Nitric oxide synthase, inducible;
    Alternative:
    Macrophage NOS;Inducible NO synthase(Inducible NOS;iNOS);Peptidyl-cysteine S-nitrosylase NOS2;NOS type II;
    Organism:
    Chicken (Gallus gallus). 
    General Annotation
    Sub Unit:
    Homodimer.
    Function:
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO may serve as both a paracrine and autocrine signal for modulating osteoclast bone resorption. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    1136
    Sequence:
    50:
    MLCPWQFAFK | PHAVKNQSSE | EKDINNNVEK | DVKVHSFVKD | DAKLHSLSKK | 
    100:
    QMKMSPIITS | AEKHPQNGIK | ASNQISRCPR | HVKVRNMENG | SSLLDTLHLT | 
    150:
    AKEVINCRTR | ACQGALMTPK | GLVRSTRDGP | VPPAELLPQA | VDFVKQYYSS | 
    200:
    FKELKIEEHL | ARLETVTKEI | ETTGTYHLTK | DELIFAAKQA | WRNAPRCIGR | 
    250:
    IQWSNLQVFD | ARDCKTAKEM | FEYICRHIQY | ATNNGNIRSA | ITIFPQRTDG | 
    300:
    KHDFRVWNSQ | LIRYAGYQMP | DGSVIGDPAS | VEFTKLCIEL | GWKPKYGRFD | 
    350:
    VVPLILQANG | QDPEIFEYPP | EIILEVPMEH | PKYEWFKELD | LKWYALPAVA | 
    400:
    NMLLEVGGLE | FTACPFNGWY | MGTEIGVRDF | CDVQRYNILK | EVGRRMGLES | 
    450:
    NKLASLWKDR | AVVEINVAVL | HSFQKQNVTI | MDHHSAAESF | MKYMQNEYRV | 
    500:
    RGGCPADWVW | IVPPMSGSIT | PVFHQEMLNY | VLTPFFYYQV | DAWKTHIWHD | 
    550:
    ETRRPKKREI | KLSILAKAVL | LASLLLQKTM | AARPKVTVIY | ATETGKSETL | 
    600:
    ANSLCSLFSC | AFNTKILCMD | EYNISDLEKE | TLLLVVTSTF | GNGDSPNNGK | 
    650:
    TLKNSLLTLK | LLRKNIRYAV | FGLGSTMYPE | FCAFAHAIDQ | KLSQLGALQL | 
    700:
    TPVGEGDELN | GQEEAFRTWA | VTAFKTACDI | FDIRGKNSIQ | LPEIYTSDDS | 
    750:
    WNPKKHRIVY | DSQTMDLTKA | LSDIHGKNVI | PMKLKFRQNL | QSLKSSRVTI | 
    800:
    LVKLSCETNQ | EVHYLPGEHI | GISPGNQPEL | VHGLIARVKD | APPADQTIRL | 
    850:
    ETCTEGGYWA | SEKKIPACTL | SQALTYLLDI | TTPPTQQLLK | KLSQLVTAEG | 
    900:
    DKQRLEVLCH | STEEYNKWKF | YNRPNILEVL | EEFPSAEVST | AFLLTQLPLL | 
    950:
    KPRYYSVSSS | CDMTPREIHL | TVAVVNYRTR | DGQGPLHHGV | CSTWLNKIAL | 
    1000:
    NETVPCFVRS | ADGFRLPKEP | AKPCILIGPG | TGIAPFRSFW | QQRLYDLEKK | 
    1050:
    GIKGGDMILL | FGCRHPDMDH | IYKEEVEEMK | RKGVLKEVFT | AYSRQPGQPK | 
    1100:
    VYVQDILQNE | LETKVCNILH | KEEGHLYVCG | DVRMARDVAQ | TLKRMLVKKL | 
    1136:
    NHTEQQAEEY | FFQLKSQKRY | HEDIFGAVFP | HEVKRI
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    Pfam:
    SMR:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    MSDS:
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    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837p
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    E0837c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    U0837m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    U0837d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    MSDS:
    Please sign in first.
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    U0837b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    U0837c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    U0837r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Chicken Nitric oxide synthase, inducible
    Cat.:
    U0837h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Polyclonal Antibody for Chicken Nitric oxide synthase, inducible
    Cat.:
    P0837Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Monoclonal Antibody for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible
    Cat.:
    R0837h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible
    Protein for Chicken Nitric oxide synthase, inducible

    R&D Technical Data
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    s

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     12% SDS-PAGE analysis

                 12% SDS-PAGE analysis

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    Citations
    [1].
    Yun-Feng Ni, Jian-Ke Kuai, Zi-Fan Lu, Guo-Dong Yang, Hai-Yan Fu, Jian Wang, Feng Tian, Xiao-Long Yan, Ya-Chao Zhao, Yun-Jie Wang, and Tao Jiang,
    References
    1. 1.
      "Molecular cloning and expression of an avian macrophage nitric-oxide synthase cDNA and the analysis of the genomic 5'-flanking region."
      Lin A.W. , Chang C.C. , McCormick C.C.
      J. Biol. Chem.271:11911-11919(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Proinflammatory agents, IL-8 and IL-10, upregulate inducible nitric oxide synthase expression and nitric oxide production in avian osteoclast-like cells."
      Sunyer T. , Rothe L. , Jiang X. , Osdoby P. , Collin-Osdoby P.
      J. Cell. Biochem.60:469-483(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-472
      tissue: Osteoclast.
    3. 3.
      "Molecular cloning and expression of inducible nitric oxide synthase in chick embryonic ventricular myocytes."
      Shimizu T. , Kinugawa K. , Sugishita Y. , Sugishita K. , Harada K. , Matsui H. , Kohmoto O. , Serizawa T. , Takahashi T.
      Cardiovasc. Res.38:405-413(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 646-986
      tissue: Heart.
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